Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- PAB7192 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7192, RRID:AB_1581244
- Product name
- TBC1D4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of TBC1D4.
- Antigen sequence
C-DDPEKIEERKKSK
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects.
Karlsson HK, Zierath JR, Kane S, Krook A, Lienhard GE, Wallberg-Henriksson H
Diabetes 2005 Jun;54(6):1692-7
Diabetes 2005 Jun;54(6):1692-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TBC1D4 polyclonal antibody (Cat # PAB7192) (0.1 ug/mL) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TBC1D4 polyclonal antibody (Cat # PAB7192, 3.8 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)