Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-20524 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPAM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is rat brain tissue lysate.
- Concentration
- 1 mg/mL
Submitted references Moderate-Intensity Exercise and High-Intensity Interval Training Affect Insulin Sensitivity Similarly in Obese Adults.
Ryan BJ, Schleh MW, Ahn C, Ludzki AC, Gillen JB, Varshney P, Van Pelt DW, Pitchford LM, Chenevert TL, Gioscia-Ryan RA, Howton SM, Rode T, Hummel SL, Burant CF, Little JP, Horowitz JF
The Journal of clinical endocrinology and metabolism 2020 Aug 1;105(8):e2941-59
The Journal of clinical endocrinology and metabolism 2020 Aug 1;105(8):e2941-59
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain tissue lysate using a GPAT1 polyclonal antibody (Product # PA5-20524) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), MSC (Lane 2), MSC differentiated to adipocytes (Lane 3) and HeLa (Lane 4). The blot was probed with Anti-GPAM Polyclonal Antibody (Product # PA5-20524, 1ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). A 94 kDa band corresponding to GPAM was observed across cell lines and tissue tested and was also enhanced in MSC after differentiating it to adipocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GPAT1 in rat brain tissue lysate with GPAM Polyclonal Antibody (Product # PA5-20524) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of GPAT1 in mouse brain tissue with GPAM Polyclonal Antibody (Product # PA5-20524) at 20 µg/mL. Green: GPAT1 Blue: DAPI staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of GPAT1 in Rat Brain tissue with GPAM Polyclonal Antibody (Product # PA5-20524) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of GPAT1 in mouse brain tissue with GPAM Polyclonal Antibody (Product # PA5-20524) at 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of GPAT1 in rat brain with GPAM Polyclonal Antibody (Product # PA5-20524) at 2.5 µg/mL.