LS-C290715

antibody from LSBio

Targeting: KCMF1 DEBT91, DKFZP434L1021, PCMF, ZZZ1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C290715

Provider

LSBio

Product name

Anti-KCMF1 Antibody (aa50-100) LS-C290715

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Bovine, Canine, Xenopus, Zebrafish

Host

Rabbit

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human and Mouse KCMF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-KCMF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human KCMF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KCMF1 antibody used for IP at 6 ug/mg lysate. KCMF1 was also immunoprecipitated by rabbit anti-KCMF1 antibody BL14690. For blotting immunoprecipitated KCMF1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.