20358-1-AP

antibody from Invitrogen Antibodies

Targeting: PRKAR1A CNC1, PRKAR1, TSE1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20358-1-AP

Provider

Invitrogen Antibodies

Product name

PRKAR1A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MESGSTAAS EEARSLRECE LYVQKHNIQA LLKDSIVQLC TARPERPMAF LREYFERLEK EEAKQIQNLQ KAGTRTDSRE DEISPPPPNP VVKGRRRRGA I (1-100 aa encoded by BC036285)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.62 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using (Product # 20358-1-AP) (PRKAR1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).