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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB1153 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1153, RRID:AB_1580533
- Product name
- SETD8 monoclonal antibody, clone 43AT551.86
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant SETD8.
- Isotype
- IgG
- Antibody clone number
- 43AT551.86
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references An miR-502-binding site single-nucleotide polymorphism in the 3'-untranslated region of the SET8 gene is associated with early age of breast cancer onset.
Product specificity and mechanism of protein lysine methyltransferases: insights from the histone lysine methyltransferase SET8.
Catalytic function of the PR-Set7 histone H4 lysine 20 monomethyltransferase is essential for mitotic entry and genomic stability.
Song F, Zheng H, Liu B, Wei S, Dai H, Zhang L, Calin GA, Hao X, Wei Q, Zhang W, Chen K
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Oct 1;15(19):6292-300
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Oct 1;15(19):6292-300
Product specificity and mechanism of protein lysine methyltransferases: insights from the histone lysine methyltransferase SET8.
Zhang X, Bruice TC
Biochemistry 2008 Jun 24;47(25):6671-7
Biochemistry 2008 Jun 24;47(25):6671-7
Catalytic function of the PR-Set7 histone H4 lysine 20 monomethyltransferase is essential for mitotic entry and genomic stability.
Houston SI, McManus KJ, Adams MM, Sims JK, Carpenter PB, Hendzel MJ, Rice JC
The Journal of biological chemistry 2008 Jul 11;283(28):19478-88
The Journal of biological chemistry 2008 Jul 11;283(28):19478-88
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The SETD8 monoclonal antibody, clone 43AT551.86.76. (Cat # MAB1153) is used in Western blot to detect SETD8 in SAOS-2 (lane 1) and NIH/3T3 cell lysates (lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TOP : Negative control of HeLa cells without PE-conjugated goat anti-mouse lgG (whole molecule). PE-conjugated goat anti-mouse lgG emits red fluorescence.Bottom : Immunofluorescence analysis of SETD8 monoclonal antibody, clone 43AT551.86.76. (Cat # MAB1153) in HeLa cells. 0.025 mg/mL primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testisreacted with SETD8 monoclonal antibody, clone 43AT551.86.76. (Cat # MAB1153), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
