LS-C818250

antibody from LSBio

Targeting: KMT5A PR-Set7, SET07, SET8, SETD8

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Enhanced validation)

Antibody Data

Product number

LS-C818250

Provider

LSBio

Product name

SETD8 / SET8 Antibody (Preservative Free) LS-C818250

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

SETD8 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 4 uL anti-SETD8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SETD8 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 54 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

SETD8 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 4 uL anti-SETD8 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SETD8 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 54 kDa.