AF3997

antibody from R&D Systems

Targeting: EIF2S1 EIF-2alpha, EIF2, EIF2A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3997

Provider

R&D Systems

Product name

Human eIF2 alpha Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat eIF2 alpha in Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q53XC0


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Silvestrol Inhibits Chikungunya Virus Replication.

Henss L, Scholz T, Grünweller A, Schnierle BS
Viruses 2018 Oct 30;10(11)

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human eIF2 alpha by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MCF-7 human breast cancer cell line, CTLL-2 mouse cytotoxic T cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eIF2 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF2 alpha at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human eIF2 alpha by Simple Western^TM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for eIF2 alpha at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eIF2 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.