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- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 18010-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#18010-1-AP, RRID:AB_2261996
- Product name
- EIF2B1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated EIF2B1 antibody (Cat. #18010-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references eIF2B conformation and assembly state regulate the integrated stress response.
Sugar phosphate activation of the stress sensor eIF2B.
Inhibition of the integrated stress response by viral proteins that block p-eIF2-eIF2B association.
eIF2B as a Target for Viral Evasion of PKR-Mediated Translation Inhibition.
Cellular eIF2B subunit localization: implications for the integrated stress response and its control by small molecule drugs.
The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.
Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.
Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.
Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.
Schoof M, Boone M, Wang L, Lawrence R, Frost A, Walter P
eLife 2021 Mar 10;10
eLife 2021 Mar 10;10
Sugar phosphate activation of the stress sensor eIF2B.
Hao Q, Heo JM, Nocek BP, Hicks KG, Stoll VS, Remarcik C, Hackett S, LeBon L, Jain R, Eaton D, Rutter J, Wong YL, Sidrauski C
Nature communications 2021 Jun 8;12(1):3440
Nature communications 2021 Jun 8;12(1):3440
Inhibition of the integrated stress response by viral proteins that block p-eIF2-eIF2B association.
Rabouw HH, Visser LJ, Passchier TC, Langereis MA, Liu F, Giansanti P, van Vliet ALW, Dekker JG, van der Grein SG, Saucedo JG, Anand AA, Trellet ME, Bonvin AMJJ, Walter P, Heck AJR, de Groot RJ, van Kuppeveld FJM
Nature microbiology 2020 Nov;5(11):1361-1373
Nature microbiology 2020 Nov;5(11):1361-1373
eIF2B as a Target for Viral Evasion of PKR-Mediated Translation Inhibition.
Wuerth JD, Habjan M, Kainulainen M, Berisha B, Bertheloot D, Superti-Furga G, Pichlmair A, Weber F
mBio 2020 Jul 14;11(4)
mBio 2020 Jul 14;11(4)
Cellular eIF2B subunit localization: implications for the integrated stress response and its control by small molecule drugs.
Hodgson RE, Varanda BA, Ashe MP, Allen KE, Campbell SG
Molecular biology of the cell 2019 Apr 1;30(8):942-958
Molecular biology of the cell 2019 Apr 1;30(8):942-958
The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.
Wong YL, LeBon L, Edalji R, Lim HB, Sun C, Sidrauski C
eLife 2018 Feb 28;7
eLife 2018 Feb 28;7
Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.
Wortham NC, Stewart JD, Harris S, Coldwell MJ, Proud CG
The Biochemical journal 2016 Mar 1;473(5):571-80
The Biochemical journal 2016 Mar 1;473(5):571-80
Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.
Sidrauski C, Tsai JC, Kampmann M, Hearn BR, Vedantham P, Jaishankar P, Sokabe M, Mendez AS, Newton BW, Tang EL, Verschueren E, Johnson JR, Krogan NJ, Fraser CS, Weissman JS, Renslo AR, Walter P
eLife 2015 Apr 15;4:e07314
eLife 2015 Apr 15;4:e07314
Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.
Wortham NC, Martinez M, Gordiyenko Y, Robinson CV, Proud CG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 May;28(5):2225-37
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 May;28(5):2225-37
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP(EIF2B1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using EIF2B1 antibody 18010-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
