18010-1-AP

antibody from Invitrogen Antibodies

Targeting: EIF2B1 EIF-2B, EIF-2Balpha, EIF2B, EIF2BA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18010-1-AP

Provider

Invitrogen Antibodies

Product name

EIF2B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MDDKELIEY FKSQMKEDPD MASAVAAIRT LLEFLKRDKG ETIQGLRANL TSAIETLCGV DSSVAVSSGG ELFLRFISLA SLEYSDYSKC KKIMIERGEL FLRRISLSRN KIADLCHTFI KDGATILTHA YSRVVLRVLE AAVAAKKRFS VYVTESQPDL SGKKMAKALC HLNVPVTVVL DAAVGYIMEK ADLVIVGAEG VVENGGIINK IGTNQMAVCA KAQNKPFYVV AESFKFVRLF PLNQQDVPDK FKYKADTLKV AQTGQDLKEE HPWVDYTAPS LITLLFTDLG VLTPSAVSDE LIKLYL (1-305 aa encoded by B C103763)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using EIF2B1 antibody 18010-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using EIF2B1 antibody 18010-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EIF2B1 (IP:18010-1-AP, 4ug; Detection:18010-1-AP 1:700) with K-562 cells lysate 3200ug.