- TA800167 - OriGene SNAI2 antibody

Submitted references Silencing of Antichondrogenic MicroRNA-221 in Human Mesenchymal Stem Cells Promotes Cartilage Repair In Vivo.

Lolli A, Narcisi R, Lambertini E, Penolazzi L, Angelozzi M, Kops N, Gasparini S, van Osch GJ, Piva R
Stem cells (Dayton, Ohio) 2016 Jul;34(7):1801-11

Slug transcription factor and nuclear Lamin B1 are upregulated in osteoarthritic chondrocytes.

Piva R, Lambertini E, Manferdini C, Capanni C, Penolazzi L, Gabusi E, Paolella F, Lolli A, Angelozzi M, Lattanzi G, Lisignoli G
Osteoarthritis and cartilage 2015 Jul;23(7):1226-30

Chondrogenic potential of Slug-depleted human mesenchymal stem cells.

Lisignoli G, Manferdini C, Lambertini E, Zini N, Angelozzi M, Gabusi E, Gambari L, Penolazzi L, Lolli A, Facchini A, Piva R
Tissue engineering. Part A 2014 Oct;20(19-20):2795-805

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Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI2 (RC202365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI2.
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800167)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800167)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800167)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800167)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800167)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800167)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Figure from citation: Immunohistochemical analysis of Slug (also known as SNAI2) on serial sections of cartilage from normal and OA cartilage by using anti-SNAI2 antibody. Bar=100 um, Inset bar=10 um. Dilution: 1:300
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: HEK293T cells transfected with either RC202365 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SNAI2 antibody(TA800167), and then analyzed by flow cytometry.
Validation comment: FC

TA800167

Antibody data