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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 12354-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VTI1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSSDFEGYE QDFAVLTAEI TSKIARVPRL PPDEKKQMVA NVEKQLEEAK ELLEQMDLEV REIPPQSRGM YSNRMRSYKQ EMGKLETDFK RSRIAYSDEV RNELLGDDGN SSENQRAHLL DNTERLERSS RRLEAGYQIA VETEQIGQEM LENLSHDREK IQRARERLRE TDANLGKSSR ILTGMLRRGC SVKKQCNLSL APKA (1-203 aa encoded by BC017052)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.28 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human lung tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12354-1-AP (VTI1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NIH/3T3 cells using 12354-1-AP (VTI1A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-VTI1A (IP:12354-1-AP, 3ug; Detection:12354-1-AP 1:1000) with MCF-7 cells lysate 520ug.
