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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30155 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL7A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, Jurkat, Raji, Neuro2A, GL261, C8D30, PC-12, Rat2.
- Concentration
- 1.02 mg/mL
Submitted references SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assemblies.
Toki N, Takahashi H, Sharma H, Valentine MNZ, Rahman FM, Zucchelli S, Gustincich S, Carninci P
Nucleic acids research 2020 Nov 18;48(20):11626-11644
Nucleic acids research 2020 Nov 18;48(20):11626-11644
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPL7A Polyclonal Antibody detects RPL7A protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.12 % SDS-PAGE. RPL7A Polyclonal Antibody (Product # PA5-30155) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPL7A Polyclonal Antibody (Product # PA5-30155). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: JurKat. Lane C: Raji. 12% SDS PAGE. RPL7A Polyclonal Antibody (Product # PA5-30155) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPL7A Polyclonal Antibody (Product # PA5-30155). Sample (30 µg of whole cell lysate). Lane A: Neuro2A. Lane B: GL261. Lane C: C8D30. 12% SDS PAGE. RPL7A Polyclonal Antibody (Product # PA5-30155) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPL7A Polyclonal Antibody detects RPL7A protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.12 % SDS-PAGE. RPL7A Polyclonal Antibody (Product # PA5-30155) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RPL7A in methanol-fixed A431 cells using a RPL7A polyclonal antibody (Product # PA5-30155) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using RPL7A (Product # PA5-30155) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
