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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- PA5-61289 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUCLG2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FGGIVNCAII ANGITKACRE LELKVPLVVR LEGTNVQEAQ KILNNSGLPI TSAIDLEDAA KKAVASVAKK
- Concentration
- 0.6 mg/mL
Submitted references Mesothelial Cell HIF1α Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk.
Hart PC, Kenny HA, Grassl N, Watters KM, Litchfield LM, Coscia F, Blaženović I, Ploetzky L, Fiehn O, Mann M, Lengyel E, Romero IL
Cell reports 2019 Dec 17;29(12):4086-4098.e6
Cell reports 2019 Dec 17;29(12):4086-4098.e6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SUCLG2 using SUCLG2 Polyclonal Antibody (Product # PA5-61289) (A) shows similar pattern to an independent SUCLG2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SUCLG2 was achieved by transfecting Hep G2 with SUCLG2 specific siRNAs (Silencer® select Products # s16774, s16775). Western blot analysis (Fig. a) was performed using whole cell extracts from the SUCLG2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with SUCLG2 Polyclonal Antibody (Product # PA5-61289, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SUCLG2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SUCLG2 Polyclonal Antibody (Product # PA5-61289) and a 45 kDa band corresponding to SUCLG2 was observed in all the tested cell models and tissue lysates. Whole cell lysates (30ug lysate) of Hep G2 (Lane 1), MOLT-4 (Lane 2), Raji (Lane 3), A549 (Lane 4), U-2 OS (Lane 5), MCF-7 (Lane 6), and Tissue extracts (30ug lysate) of Mouse Liver (Lane 7), Mouse Kidney (Lane 8), Mouse Brain (Lane 9) and Mouse Ovary (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SUCLG2 in human cell line Hep G2 shows positivity in mitochondria. Samples were probed using a SUCLG2 Polyclonal Antibody (Product # PA5-61289).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SUCLG2 in human testis using SUCLG2 Polyclonal Antibody (Product # PA5-61289).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SUCLG2 in human liver using SUCLG2 Polyclonal Antibody (Product # PA5-61289).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SUCLG2 in human kidney, liver, pancreas and testis using SUCLG2 Polyclonal Antibody (Product # PA5-61289) (A) shows similar protein distribution across tissues to an independent SUCLG2 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SUCLG2 in human pancreas using SUCLG2 Polyclonal Antibody (Product # PA5-61289) shows weak to moderate positivity in mitochondria in exocrine glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SUCLG2 in human kidney using SUCLG2 Polyclonal Antibody (Product # PA5-61289) shows strong positivity in mitochondria in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SUCLG2 in human colon using SUCLG2 Polyclonal Antibody (Product # PA5-61289) shows strong positivity in mitochondria in glandular cells.
