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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB1774 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1774, RRID:AB_1238140
- Product name
- UBA3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of UBA3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The covalent modifier Nedd8 is critical for the activation of Smurf1 ubiquitin ligase in tumorigenesis.
Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer.
Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.
Xie P, Zhang M, He S, Lu K, Chen Y, Xing G, Lu Y, Liu P, Li Y, Wang S, Chai N, Wu J, Deng H, Wang HR, Cao Y, Zhao F, Cui Y, Wang J, He F, Zhang L
Nature communications 2014 May 13;5:3733
Nature communications 2014 May 13;5:3733
Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Walden H, Podgorski MS, Schulman BA
Nature 2003 Mar 20;422(6929):330-4
Nature 2003 Mar 20;422(6929):330-4
Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer.
Bohnsack RN, Haas AL
The Journal of biological chemistry 2003 Jul 18;278(29):26823-30
The Journal of biological chemistry 2003 Jul 18;278(29):26823-30
Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.
Gong L, Yeh ET
The Journal of biological chemistry 1999 Apr 23;274(17):12036-42
The Journal of biological chemistry 1999 Apr 23;274(17):12036-42
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The UBA3 polyclonal antibody (Cat # PAB1774) is used in Western blot to detect UBA3 in mouse brain tissue lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with UBA3 polyclonal antibody (Cat # PAB1774) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
