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LearnBSM-54417R
antibody from Invitrogen Antibodies
Targeting: ADAMTS13
C9orf8, DKFZp434C2322, FLJ42993, MGC118899, MGC118900, TTP, vWF-CP, VWFCP
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- BSM-54417R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAMTS13 Monoclonal Antibody (1G9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody recognizes the ~90 kDa (apparent MW) MDM2 protein. Also recognizes isoforms at ~57 and ~74/76 kDa. The epitope recognized by this antibody is located within amino acids 26-169 of the human protein. Positive controls: OsA-CL, MCF-7, HeLa cell lysates. HOS cells can be used for negative control. Staining of formalin fixed, paraffin embedded tissue requires heat induced epitope retrieval pretreatment.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 1G9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: SiHa Cells; Lane 2: PC-3M Cells; Lane 3: A549 Cells; Probed with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells with ADAMTS13 (1G9) Monoclonal Antibody (bsm-54417R) at a 1:100 dilution (purple) compared with an unlabeled control (cells with out incubation with primary antibody; yellow).
