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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 23350-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRIA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: YSRGVFAIF GLYDKRSVHT LTSFCSALHI SLITPSFPTE GESQFVLQLR PSLRGALLSL LDHYEWNCFV FLYDTDRGYS ILQAIMEKAG QNGWHVSAIC VENFNDVSYR QLLEELDRRQ EKKFVIDCEI ERLQNILEQI VSVG (87-229 aa encoded by BC167786)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 23350-1-AP ( GRIA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 23350-1-AP (GRIA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 23350-1-AP (GRIA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 23350-1-AP ( GRIA4 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 23350-1-AP ( GRIA4 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 SH-SY5Y cells were stained with 0.2ug GRIA4 antibody (23350-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GRIA4 (IP:23350-1-AP, 4ug; Detection:23350-1-AP 1:300) with mouse brain tissue lysate 4000ug.
