PA1-31405

CD36L1, CLA-1, CLA1, SR-BI, SRB1

Antibody data

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Validation data

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SR-BI in human adrenal cell lysate using a SR-BI polyclonal antibody (Product # PA1-31405).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins using a SR-BI polyclonal antibody (Product # PA1-31405).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SR-BI was performed by loading 25 µg of mouse liver tissue lysates. Proteins were transferred to a membrane and probed with a SR-BI Polyclonal Antibody (Product # PA1-31405) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SR-BI was performed by loading HeLa cell lysate. Proteins were transferred to a membrane and probed with a SR-BI Polyclonal Antibody (Product # PA1-31405).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SR-BI was performed by loading human adrenal tissue. Proteins were transferred to a membrane and probed with a SR-BI Polyclonal Antibody (Product # PA1-31405).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using anti-SR-BI Polyclonal Antibody (Product # PA1-31405) and a 75 kDa band corresponding to SR-BI was high in Hep G2 when compared to Jurkat; Mouse Liver, Rat Liver and Mouse Testis when compared to Mouse Kidney and Mouse Brain as reported in literatures. Uncharacterized bands (*) were also observed between 30 kDa- 60 kDa across samples tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), Jurkat (Lane 3), Mouse Liver (Lane 4), Rat Liver (Lane 5), Mouse Testis (Lane 6), Mouse Kidney (Lane 7) and Mouse Brain (Lane 8) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).