PA1-31405

antibody from Invitrogen Antibodies

Targeting: SCARB1 CD36L1, CLA-1, CLA1, SR-BI, SRB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-31405

Provider

Invitrogen Antibodies

Product name

SR-BI Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-31405 detects SR-BI from rat, mouse, human samples.

Reactivity

Human, Mouse, Rat, Hamster, Rabbit

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SR-BI in rat H4IIE total cell lysates and plasma membrane proteins using a SR-BI polyclonal antibody (Product # PA1-31405).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SR-BI was performed by loading 25 µg of mouse liver tissue lysates. Proteins were transferred to a membrane and probed with a SR-BI Polyclonal Antibody (Product # PA1-31405) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SR-BI was performed by loading HeLa cell lysate. Proteins were transferred to a membrane and probed with a SR-BI Polyclonal Antibody (Product # PA1-31405).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SR-BI was performed by loading human adrenal tissue. Proteins were transferred to a membrane and probed with a SR-BI Polyclonal Antibody (Product # PA1-31405).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using anti-SR-BI Polyclonal Antibody (Product # PA1-31405) and a 75 kDa band corresponding to SR-BI was high in Hep G2 when compared to Jurkat; Mouse Liver, Rat Liver and Mouse Testis when compared to Mouse Kidney and Mouse Brain as reported in literatures. Uncharacterized bands (*) were also observed between 30 kDa- 60 kDa across samples tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), Jurkat (Lane 3), Mouse Liver (Lane 4), Rat Liver (Lane 5), Mouse Testis (Lane 6), Mouse Kidney (Lane 7) and Mouse Brain (Lane 8) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SR-BI in HeLa cells using a SR-BI polyclonal antibody (Product # PA1-31405) at a dilution of 1:50 (green). Nuclei and actin were counterstained with DAPI (blue) and a Phalloidin (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SR-BI in human fibroblasts. Cells were fixed with 4% PFA, permeabilized with 0.2% Tween and SR-BI Polyclonal Antibody (Product # PA1-31405) (Green) at a dilution of 1:100 was used as a primary antibody. Blue: DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SR-BI in HeLa cells using SR-BI Polyclonal Antibody (Product # PA1-31405) (Green) at a dilution of 2 µg/mL. Red : Tubulin. Blue: DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SR-BI in human adrenal cortex tissue using SR-BI Polyclonal Antibody (Product # PA1-31405).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SR-BI in mouse liver tissue using SR-BI Polyclonal Antibody (Product # PA1-31405) at a dilution of 1:300.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SR-BI in mouse liver tissue using SR-BI Polyclonal Antibody (Product # PA1-31405) at a dilution of 1:300.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of SR-BI in HepG2 cells using a SR-BI polyclonal antibody (Product # PA1-31405).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of TLR7 was performed in HeLa cells (fixed with 4% PFA, permeabilized with 0.1% saponin) using a TLR7 Polyclonal Antibody (Product # PA1-28109) (Blue) at a dilution of 2 µg/mL. Orange : isotype control.