11280-1-AP

antibody from Invitrogen Antibodies

Targeting: EIF4A2 BM-010, DDX2B, EIF4A, EIF4F

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11280-1-AP

Provider

Invitrogen Antibodies

Product name

EIF4A2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: APTRELAQQ IQKVILALGD YMGATCHACI GGTNVRNEMQ KLQAEAPHIV VGTPGRVFDM LNRRYLSPKW IKMFVLDEAD EMLSRGFKDQ IYEIFQKLNT SIQVVLLSAT MPTDVLEVTK KFMRDPIRIL VKKEELTLEG IKQFYINVER EEWKLDTLCD LYETLTITQA VIFLNTRRKV DWLTEKMHAR DFTVSALHGD MDQKERDVIM REFRSGSSRV LITTDLLARG IDVQQVSLVI NYDLPTNREN YIHRIGRGGR FGRKGVAINF VTEEDKRILR DIETFYNTTV EEMPMNVADL I (108-407 aa encoded by BC015842)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 11280-1-AP (EIF4A2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 11280-1-AP (EIF4A2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 11280-1-AP (EIF4A2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 11280-1-AP (EIF4A2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 11280-1-AP) (EIF4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EIF4A2 (IP:11280-1-AP, 4ug; Detection:11280-1-AP 1:500) with Jurkat cells lysate 4000ug.