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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-12900 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Kir2.1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 45kDa. No cross-reactivity against Kir2.2 or Kir2.3.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Kir2.1 Antibody (S112B-14) [NBP2-12900] - analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112B-14 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Kir2.1 Antibody (S112B-14) [NBP2-12900] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.
