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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00051255-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00051255-M01, RRID:AB_2180117
- Product name
- Mouse Monoclonal RNF181 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. LOC51255 - hypothetical protein LOC51255
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF181 Antibody (5A7) [H00051255-M01] - Analysis of RNF181 expression in transfected 293T cell line by RNF181 monoclonal antibody (M01), clone 5A7.Lane 1: RNF181 transfected lysate(17.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF181 Antibody (5A7) [H00051255-M01] - RNF181 monoclonal antibody (M01), clone 5A7 Analysis of RNF181 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RNF181 Antibody (5A7) [H00051255-M01] - Detection limit for recombinant GST tagged RNF181 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RNF181 Antibody (5A7) [H00051255-M01] - Analysis of RNF181 transfected lysate using anti-RNF181 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RNF181 monoclonal antibody.
