PA5-30170

antibody from Invitrogen Antibodies

Targeting: TRA2B Htra2-beta, PPP1R156, SFRS10

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30170

Provider

Invitrogen Antibodies

Product name

SFRS10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: JurKat.

Concentration

0.41 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SFRS10 Polyclonal Antibody (Product # PA5-30170). Sample (30 µg of whole cell lysate). Lane A: JurKat. 12% SDS PAGE. SFRS10 Polyclonal Antibody (Product # PA5-30170) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of SFRS10 was achieved by transfecting HeLa with SFRS10 specific siRNAs (Silencer® select Product # s12750, s12749). Western blot analysis (Fig. a) was performed using whole cell extracts from the SFRS10 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with SFRS10 Polyclonal Antibody (Product # PA5-30170, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SFRS10.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on modified whole cell lysates (1% SDS) of Jurkat (Lane 1), U-87 MG (Lane 2), HeLa (Lane 3), SK-OV-3 (Lane 4), PC-3 (Lane 5), DU 145 (Lane 6) and MCF-7 (Lane 7). The blot was probed with SRSF10 Polyclonal Antibody (Product # PA5-30170, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 39 kDa band corresponding to SRSF10 was observed across the panel tested.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using TRA2B (Product # PA5-30170) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SFRS10 Polyclonal Antibody detects TRA2B protein at nucleus on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. SFRS10 Polyclonal Antibody (Product # PA5-30170) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SFRS10 Polyclonal Antibody detects TRA2B protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SFRS10 Polyclonal Antibody (Product # PA5-30170) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.