PA5-28808

antibody from Invitrogen Antibodies

Targeting: ADRB1 ADRB1R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28808

Provider

Invitrogen Antibodies

Product name

beta-1 Adrenergic Receptor Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A431, H1299.

Concentration

1 mg/mL

References

Submitted references

DMD Pluripotent Stem Cell Derived Cardiac Cells Recapitulate in vitro Human Cardiac Pathophysiology.

Jelinkova S, Vilotic A, Pribyl J, Aimond F, Salykin A, Acimovic I, Pesl M, Caluori G, Klimovic S, Urban T, Dobrovolna H, Soska V, Skladal P, Lacampagne A, Dvorak P, Meli AC, Rotrekl V
Frontiers in bioengineering and biotechnology 2020;8:535

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Glucagon-Like Peptide 1 Receptor Activation Augments Cardiac Output and Improves Cardiac Efficiency in Obese Swine After Myocardial Infarction.

Sassoon DJ, Tune JD, Mather KJ, Noblet JN, Eagleson MA, Conteh AM, Sturek JT, Goodwill AG
Diabetes 2017 Aug;66(8):2230-2240

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The epicardium as modulator of the cardiac autonomic response during early development.

Kelder TP, Duim SN, Vicente-Steijn R, Végh AM, Kruithof BP, Smits AM, van Bavel TC, Bax NA, Schalij MJ, Gittenberger-de Groot AC, DeRuiter MC, Goumans MJ, Jongbloed MR
Journal of molecular and cellular cardiology 2015 Dec;89(Pt B):251-9

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using beta-1 Adrenergic Receptor Polyclonal Antibody (Product # PA5-28808). Sample (30 µg of whole cell lysate). A: A431. B: H1299. 12% SDS PAGE. Beta-1 Adrenergic Receptor Polyclonal Antibody (Product # PA5-28808) diluted at 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-ADRB1 Rabbit Polyclonal Antibody (Product # PA5-28808) and a 51 kDa bands corresponding to ADRB1 was observed in the positive tissue, Mouse and Rat brain whereas non specific bands were observed in the negative tissues, Mouse and Rat lung. Whole cell extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Mouse lung (Lane 3) or Rat lung (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of beta 1 Adrenergic Receptor in methanol-fixed A431 cells using a beta 1 Adrenergic Receptor polyclonal antibody (Product # PA5-28808) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded HUH-7 xenograft, using Adrenergic receptor beta 1 (Product # PA5-28808) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.