A305-855A-T

antibody from Invitrogen Antibodies

Targeting: SARDH DMGDHL1, SDH

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-855A-T

Provider

Invitrogen Antibodies

Product name

SARDH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SARDH by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, K-562, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SARDH antibody A305-855A-M (lot A305-855A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SARDH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SARDH antibody (Product # A305-855A-T; lot A305-855A-T-1) used for IP at 100 µL per reaction. SARDH was also immunoprecipitated by rabbit anti-SARDH antibody (Product # A305-856A-T). For blotting immunoprecipitated SARDH, Product # A305-855A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.