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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- bs-3080R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-3080R, RRID:AB_10853520
- Product name
- CYLD (Ser418) Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic phosphopeptide derived from human CYLD around the phosphorylation site of Ser418
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
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Supportive validation
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- Bioss (provider)
- Main image
- Experimental details
- Lane 1: rat brain lysates Lane 2: human gastric carcinoma lysates probed with Anti phospho-CYLD(Ser418)Polyclonal Antibody, Unconjugated (bs-3080R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 105kD. Observed band size: 105kD.
- Sample type
- Human
- Other comments
- Stomach
