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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-34630 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYLD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HepG2, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12.
- Concentration
- 1 mg/mL
Submitted references Cylindromatosis Is Required for Survival of a Subset of Melanoma Cells.
La T, Jin L, Liu XY, Song ZH, Farrelly M, Feng YC, Yan XG, Zhang YY, Thorne RF, Zhang XD, Teng L
Oncology research 2020 Sep 1;28(4):385-398
Oncology research 2020 Sep 1;28(4):385-398
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CYLD was performed by separating 30 µg of HepG2 lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a CYLD Polyclonal Antibody (Product # PA5-34630) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CYLD was performed by separating 30 µg of various whole cell lysates by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CYLD Polyclonal Antibody (Product # PA5-34630) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A. C8D30, B. NIH-3T3 , C. BCL-1 , D. Raw264.7 , E. C2C12.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CYLD was performed by separating 50 µg of rat brain extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CYLD Polyclonal Antibody (Product # PA5-34630) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of paraformaldehyde-fixed A431 cells using anti-CYLD Polyclonal Antibody (Product # PA5-34630) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CYLD in paraformaldehyde-fixed A431 cells using CYLD Polyclonal Antibody (Product # PA5-34630) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CYLD was performed in paraffin-embedded human gastric cancer tissue using CYLD Polyclonal Antibody (Product # PA5-34630) at a dilution of 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
