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- Antibody Data
- Antigen structure
- References [18]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 17721-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#17721-1-AP, RRID:AB_2092506
- Product name
- DHX9 antibody
- Antibody type
- Polyclonal
- Description
- DHX9 antibody (Cat. #17721-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references SNORA42 promotes oesophageal squamous cell carcinoma development through triggering the DHX9/p65 axis.
RNA pull-down confocal nanoscanning (RP-CONA) detects quercetin as pri-miR-7/HuR interaction inhibitor that decreases α-synuclein levels.
Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.
Interaction of 7SK with the Smn complex modulates snRNP production.
Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.
LINC00460/DHX9/IGF2BP2 complex promotes colorectal cancer proliferation and metastasis by mediating HMGA1 mRNA stability depending on m6A modification.
High expression of DHX9 promotes the growth and metastasis of hepatocellular carcinoma.
SPOP attenuates migration and invasion of choriocarcinoma cells by promoting DHX9 degradation.
R-loops coordinate with SOX2 in regulating reprogramming to pluripotency.
Splicing Regulator p54nrb /Non-POU Domain-Containing Octamer-Binding Protein Enhances Carcinogenesis Through Oncogenic Isoform Switch of MYC Box-Dependent Interacting Protein 1 in Hepatocellular Carcinoma.
Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.
Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.
Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1.
RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination.
Minichromosome maintenance protein 2 and 3 promote osteosarcoma progression via DHX9 and predict poor patient prognosis.
Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Interacts with Nsp9 and Cellular DHX9 To Regulate Viral RNA Synthesis.
Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation.
Lin28a regulates neuronal differentiation and controls miR-9 production.
Shan Y, Wei S, Xiang X, Dai S, Cui W, Zhao R, Zhang C, Zhang W, Zhao L, Shan B
Genomics 2021 Sep;113(5):3015-3029
Genomics 2021 Sep;113(5):3015-3029
RNA pull-down confocal nanoscanning (RP-CONA) detects quercetin as pri-miR-7/HuR interaction inhibitor that decreases α-synuclein levels.
Zhu S, Choudhury NR, Rooney S, Pham NT, Koszela J, Kelly D, Spanos C, Rappsilber J, Auer M, Michlewski G
Nucleic acids research 2021 Jun 21;49(11):6456-6473
Nucleic acids research 2021 Jun 21;49(11):6456-6473
Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.
Khan M, Hou S, Azam S, Lei H
Nucleic acids research 2021 Jun 21;49(11):6420-6436
Nucleic acids research 2021 Jun 21;49(11):6420-6436
Interaction of 7SK with the Smn complex modulates snRNP production.
Ji C, Bader J, Ramanathan P, Hennlein L, Meissner F, Jablonka S, Mann M, Fischer U, Sendtner M, Briese M
Nature communications 2021 Feb 24;12(1):1278
Nature communications 2021 Feb 24;12(1):1278
Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.
Ma Y, Liu S, Gao J, Chen C, Zhang X, Yuan H, Chen Z, Yin X, Sun C, Mao Y, Zhou F, Shao Y, Liu Q, Xu J, Cheng L, Yu D, Li P, Yi P, He J, Geng G, Guo Q, Si Y, Zhao H, Li H, Banes GL, Liu H, Nakamura Y, Kurita R, Huang Y, Wang X, Wang F, Fang G, Engel JD, Shi L, Zhang YE, Yu J
Developmental cell 2021 Feb 22;56(4):478-493.e11
Developmental cell 2021 Feb 22;56(4):478-493.e11
LINC00460/DHX9/IGF2BP2 complex promotes colorectal cancer proliferation and metastasis by mediating HMGA1 mRNA stability depending on m6A modification.
Hou P, Meng S, Li M, Lin T, Chu S, Li Z, Zheng J, Gu Y, Bai J
Journal of experimental & clinical cancer research : CR 2021 Feb 1;40(1):52
Journal of experimental & clinical cancer research : CR 2021 Feb 1;40(1):52
High expression of DHX9 promotes the growth and metastasis of hepatocellular carcinoma.
Shi F, Cao S, Zhu Y, Yu Q, Guo W, Zhang S
Journal of clinical laboratory analysis 2021 Dec;35(12):e24052
Journal of clinical laboratory analysis 2021 Dec;35(12):e24052
SPOP attenuates migration and invasion of choriocarcinoma cells by promoting DHX9 degradation.
Yuan D, Chen Y, Yang Z, Li G, Wu M, Jiang J, Li D, Yu Q
American journal of cancer research 2020;10(8):2428-2445
American journal of cancer research 2020;10(8):2428-2445
R-loops coordinate with SOX2 in regulating reprogramming to pluripotency.
Li Y, Song Y, Xu W, Li Q, Wang X, Li K, Wang J, Liu Z, Velychko S, Ye R, Xia Q, Wang L, Guo R, Dong X, Zheng Z, Dai Y, Li H, Yao M, Xue Y, Schöler HR, Sun Q, Yao H
Science advances 2020 Jun;6(24):eaba0777
Science advances 2020 Jun;6(24):eaba0777
Splicing Regulator p54nrb /Non-POU Domain-Containing Octamer-Binding Protein Enhances Carcinogenesis Through Oncogenic Isoform Switch of MYC Box-Dependent Interacting Protein 1 in Hepatocellular Carcinoma.
Hu Z, Dong L, Li S, Li Z, Qiao Y, Li Y, Ding J, Chen Z, Wu Y, Wang Z, Huang S, Gao Q, Zhao Y, He X
Hepatology (Baltimore, Md.) 2020 Aug;72(2):548-568
Hepatology (Baltimore, Md.) 2020 Aug;72(2):548-568
Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.
Xu S, Zhan M, Jiang C, He M, Yang L, Shen H, Huang S, Huang X, Lin R, Shi Y, Liu Q, Chen W, Mohan M, Wang J
Nature communications 2019 Dec 2;10(1):5492
Nature communications 2019 Dec 2;10(1):5492
Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.
Azam S, Hou S, Zhu B, Wang W, Hao T, Bu X, Khan M, Lei H
RNA biology 2019 Aug;16(8):1001-1009
RNA biology 2019 Aug;16(8):1001-1009
Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1.
Kooshapur H, Choudhury NR, Simon B, Mühlbauer M, Jussupow A, Fernandez N, Jones AN, Dallmann A, Gabel F, Camilloni C, Michlewski G, Caceres JF, Sattler M
Nature communications 2018 Jun 26;9(1):2479
Nature communications 2018 Jun 26;9(1):2479
RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination.
Choudhury NR, Heikel G, Trubitsyna M, Kubik P, Nowak JS, Webb S, Granneman S, Spanos C, Rappsilber J, Castello A, Michlewski G
BMC biology 2017 Nov 8;15(1):105
BMC biology 2017 Nov 8;15(1):105
Minichromosome maintenance protein 2 and 3 promote osteosarcoma progression via DHX9 and predict poor patient prognosis.
Cheng DD, Zhang HZ, Yuan JQ, Li SJ, Yang QC, Fan CY
Oncotarget 2017 Apr 18;8(16):26380-26393
Oncotarget 2017 Apr 18;8(16):26380-26393
Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Interacts with Nsp9 and Cellular DHX9 To Regulate Viral RNA Synthesis.
Liu L, Tian J, Nan H, Tian M, Li Y, Xu X, Huang B, Zhou E, Hiscox JA, Chen H
Journal of virology 2016 Jun 1;90(11):5384-5398
Journal of virology 2016 Jun 1;90(11):5384-5398
Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation.
Choudhury NR, Nowak JS, Zuo J, Rappsilber J, Spoel SH, Michlewski G
Cell reports 2014 Nov 20;9(4):1265-72
Cell reports 2014 Nov 20;9(4):1265-72
Lin28a regulates neuronal differentiation and controls miR-9 production.
Nowak JS, Choudhury NR, de Lima Alves F, Rappsilber J, Michlewski G
Nature communications 2014 Apr 11;5:3687
Nature communications 2014 Apr 11;5:3687
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17721-1-AP(DHX9 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using DHX9 antibody 17721-1-AP at 1:100 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human brain using 17721-1-AP(DHX9 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human brain using 17721-1-AP(DHX9 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
