RQ5970

antibody from NSJ Bioreagents

Targeting: DHX9 DDX9, LKP, RHA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5970

Provider

NSJ Bioreagents

Product name

DHX9 Antibody / RNA Helicase A

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the RNA Helicase A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) HEK293, 3) Jurkat, 4) K562, 5) Caco-2, 6) SW620, 7) Raji and 8) A549 lysate with RNA Helicase A antibody. Predicted molecular weight ~140 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat PC-12, 2) mouse brain and 3) mouse RAW264.7 lysate with RNA Helicase A antibody. Predicted molecular weight ~140 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human gastric cancer with RNA Helicase A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with RNA Helicase A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human renal cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse liver with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse liver with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HL60 cells with RNA Helicase A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNA Helicase A antibody.