NB110-40579

antibody from Novus Biologicals

Targeting: DHX9 DDX9, LKP, RHA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB110-40579

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-40579, RRID:AB_669072

Product name

Rabbit Polyclonal RNA Helicase A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Antigen sequence

The epitope recognized by NB 110-40
578 maps to a region between residu
e 1220 and the C-terminus (residue
1270) of human DEAH (Asp-Glu-Ala-Hi
s) Box Polypeptide 9 using the numb
ering given in entry CAA71668.1 (Ge
neID 1660).


Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Poxviral protein E3-altered cytokine production reveals that DExD/H-box helicase 9 controls Toll-like receptor-stimulated immune responses.

Dempsey A, Keating SE, Carty M, Bowie AG
The Journal of biological chemistry 2018 Sep 28;293(39):14989-15001

---

The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition.

Moldovan JB, Moran JV
PLoS genetics 2015 May;11(5):e1005121

---

Chromatin context and ncRNA highlight targets of MeCP2 in brain.

Maxwell SS, Pelka GJ, Tam PP, El-Osta A
RNA biology 2013 Nov;10(11):1741-57

---

Identification of small molecules that suppress microRNA function and reverse tumorigenesis.

Watashi K, Yeung ML, Starost MF, Hosmane RS, Jeang KT
The Journal of biological chemistry 2010 Aug 6;285(32):24707-16

---

Identification of RNA helicase A as a new host factor in the replication cycle of foot-and-mouth disease virus.

Lawrence P, Rieder E
Journal of virology 2009 Nov;83(21):11356-66

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 on HeLa whole cell lystate using NB110-40579.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: RNA Helicase A Antibody [NB110-40579] - Detection of human DHX9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 (lot NB110-40579-2) used for IP at 6 ug per reaction. DHX9 was also immunoprecipitated by a previous lot of this antibody NB110-40579-1 For blotting immunoprecipitated DHX9, NB110-40579 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: RNA Helicase A Antibody [NB110-40579] - FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-DHX9 used at a dilution of 1:200 (1 ug/mL). Detection: DAB