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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PB9902 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TRPM4 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for TRPM4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. TRPM4 information: Molecular Weight: 134301 MW; Subcellular Localization: Isoform 1: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum. Golgi apparatus; Tissue Specificity: Widely expressed with a high expression in intestine and prostate. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells. Prominently expressed in Purkinje fibers. Expressed at higher levels in T-helper 2 (Th2) cells as compared to T-helper 1 (Th1) cells.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of TRPM4 expression in mouse spleen extract (lane 1) and SW620 whole cell lysates (lane 2). TRPM4 at 134KD was detected using rabbit anti- TRPM4 Antigen Affinity purified polyclonal antibody (Catalog # PB9902) at0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image
