MA5-27733

antibody from Invitrogen Antibodies

Targeting: BMP8B BMP8, OP-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27733

Provider

Invitrogen Antibodies

Product name

BMP-8B Monoclonal Antibody (GT1017)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: human BMP8B-transfected 293T cells

Antibody clone number

GT1017

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BMP-8B in A) 293T whole cell lysate, B) 30 whole cell lysate of Myc-DDK-human BMP8B-transfected 293T cells using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with BMP-8B monoclonal antibody (Product # MA5-27733) using a dilution of 1:20,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BMP-8B Monoclonal Antibody (GT1017) detects BMP8B protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 whole cell lysate/extract of Myc-DDK-human BMP8B-transfected 293T cells.10 % SDS-PAGE. BMP-8B Monoclonal Antibody (GT1017) (Product # MA5-27733) dilution: 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BMP-8B Monoclonal Antibody (GT1017) detects BMP8B protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 whole cell lysate/extract of Myc-DDK-human BMP8B-transfected 293T cells.10 % SDS-PAGE. BMP-8B Monoclonal Antibody (GT1017) (Product # MA5-27733) dilution: 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of BMP-8B was performed in Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min. Green: BMP-8B Monoclonal Antibody (GT1017) (Product # MA5-27733) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of BMP-8B was performed in Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min. Green: BMP-8B Monoclonal Antibody (GT1017) (Product # MA5-27733) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.