A700-069

antibody from Invitrogen Antibodies

Targeting: BRD3 KIAA0043, ORFX, RING3L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-069

Provider

Invitrogen Antibodies

Product name

BRD3 Recombinant Rabbit Monoclonal Antibody (BLR069G)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR069G

Vial size

100 µL

Concentration

100 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD3 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, RKO, Jurkat, LNCaP, HeLa, MCF-7, K-562, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD3 recombinant monoclonal antibody [BLR069G] (Product # A700-069 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD3 by immunocytochemistry.Sample: FFPE section of OVCAR-3 cells.Antibody: Rabbit anti-BRD3 recombinant monoclonal antibody [BLR069G] (Product # A700-069; lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD3 by immunohistochemistry. AntibodySample: FFPE section of lung carcinoma.Antibody: Rabbit anti-BRD3 recombinant monoclonal antibody (BLR069G) (A700-069 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-BRD3 recombinant monoclonal antibody [BLR069G] (Product # A700-069 lot 1) used at 1:200. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG (A120-101D4). Counterstain: DAPI (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD3 recombinant monoclonal antibody [BLR069G] (Product # A700-069 lot 1) used for IP at 20 µL/mg lysate. BRD3 was also immunoprecipitated by rabbit anti-BRD3 antibodies (Product# A302-367A and Product # A302-368A). For blotting immunoprecipitated BRD3 (Product # A700-069) was used at 1:1000. Chemiluminescence with an exposure time of 1 second.