Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28761 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPRC5C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of GPCR5C using 30 µg of HCT116 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GPCR5C polyclonal antibody (Product # PA5-28761) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot using GPRC5C Polyclonal Antibody (Product # PA5-28761). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5C Polyclonal Antibody (Product # PA5-28761) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GPRC5C Polyclonal Antibody detects GPRC5C protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. GPRC5C stained by GPRC5C Polyclonal Antibody (Product # PA5-28761) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.