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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PAB14068 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14068, RRID:AB_10677216
- Product name
- Wdr5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Wdr5.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot was performed on whole cell lysates from mouse fibroblasts (Lane 1, NIH/3T3) and embryonic stem cells (Lane 2, E14Tg2a) with Wdr5 polyclonal antibody (Cat # PAB14068), diluted 1 : 1,000 in BSA/PBS-Tween.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of Wdr5 polyclonal antibody (Cat # PAB14068). By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 12,300.
- Validation comment
- ELISA
