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- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- AF826 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Caspase-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse Caspase-2 precursor and the small Caspase-2 subunit that is generated during proteolytic activation in Western blots.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 80 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells.
The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
Bax translocation is crucial for the sensitivity of leukaemic cells to etoposide-induced apoptosis.
Danciu C, Zupko I, Bor A, Schwiebs A, Radeke H, Hancianu M, Cioanca O, Alexa E, Oprean C, Bojin F, Soica C, Paunescu V, Dehelean CA
International journal of molecular sciences 2018 Nov 16;19(11)
International journal of molecular sciences 2018 Nov 16;19(11)
The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9982-7
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9982-7
Bax translocation is crucial for the sensitivity of leukaemic cells to etoposide-induced apoptosis.
Jia L, Patwari Y, Srinivasula SM, Newland AC, Fernandes-Alnemri T, Alnemri ES, Kelsey SM
Oncogene 2001 Aug 9;20(35):4817-26
Oncogene 2001 Aug 9;20(35):4817-26
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human and Mouse Caspase-2 Precursor and Small Subunit by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line treated with 1 μM staurosporine (STS) for the indicated times and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.8 µg/mL of Rabbit Anti-Human/Mouse Caspase-2 Polyclonal Antibody (Catalog # AF826), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Caspase-2 precursor and small subunit at approximately 43-54 kDa and 13 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
