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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- 600-401-I17 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-I17, RRID:AB_2614314
- Product name
- Anti-SMO (RABBIT) Antibody - 600-401-I17
- Antibody type
- Polyclonal
- Description
- Anti-SMO Antibody was purified by Antigen affinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-SMO from Human, Mouse, New World monkey, Rat and Sheep based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-SMO from other sources has not been determined.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.5 mg/ml
- Storage
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Handling
- Dry Ice
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Immunohistochemistry of Rabbit Anti-SMO antibody. Tissue: human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: SMO antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: SMO is a multi-pass membrane protein. Staining: An isotype control antibody is located above the SMO antibody on the bottom image which is stained brown.
- Validation comment
- Immunohistochemistry
