Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1508 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Soggy-1/DkkL1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Soggy-1/DkkL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human Soggy-1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAF02679- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references SLXL1, a novel acrosomal protein, interacts with DKKL1 and is involved in fertilization in mice.
DkkL1 (Soggy), a Dickkopf family member, localizes to the acrosome during mammalian spermatogenesis.
Zhuang XJ, Hou XJ, Liao SY, Wang XX, Cooke HJ, Zhang M, Han C
PloS one 2011;6(6):e20866
PloS one 2011;6(6):e20866
DkkL1 (Soggy), a Dickkopf family member, localizes to the acrosome during mammalian spermatogenesis.
Kohn MJ, Kaneko KJ, DePamphilis ML
Molecular reproduction and development 2005 Aug;71(4):516-22
Molecular reproduction and development 2005 Aug;71(4):516-22
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Soggy-1/DkkL1 by Western Blot. Western blot shows lysate of mouse testis tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Soggy-1/DkkL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Soggy-1/DkkL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
