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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX133148 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX133148, RRID:AB_2801548
- Product name
- VGluT1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with vGluT1 antibody (GTX133148) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VGluT1 antibody detects VGluT1 protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: VGluT1 protein stained by VGluT1 antibody (GTX133148) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VGluT1 antibody detects VGluT1 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: VGluT1 protein stained by VGluT1 antibody (GTX133148) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VGluT1 antibody detects VGluT1 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. VGluT1 antibody (GTX133148) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VGluT1 antibody detects VGluT1 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. VGluT1 antibody (GTX133148) diluted at 1:500.
