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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
- Immunohistochemistry [6]
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- Product number
- STJ13100471 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-VGluT1 antibody (2-50) (STJ13100471)
- Antibody type
- Polyclonal
- Description
- Nz White Rabbit polyclonal antibody anti-VGluT1 (2-50) is suitable for use in Western Blot, Immunohistochemistryr, Flow Cytometry, Immunocytochemistry and Immunofluorescence research applications.
- Reactivity
- Human, Mouse, Rat
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- WB on rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1: 6000 incubated at 4C overnight.1
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50) : STJ13100471.HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT ModuleBlocking: 0.2% LFDM in TBST filtered thru 0.2 umDetection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 2000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50) : STJ13100471.HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 2000, incubated 30 min at RT (using Autostainer) . Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50) : STJ13100471.HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 2000, incubated 30 min at RT (using Autostainer) . Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50) : STJ13100471.HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 2000, incubated 30 min at RT (using Autostainer) . Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50) : STJ13100471.HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 2000, incubated 30 min at RT (using Autostainer) . Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- IHC on paraffin sections of rat spinal cord using Rabbit antibody to VGluT1 (2-50) : STJ13100471.HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 2000, incubated 30 min at RT (using Autostainer) . Sections were counterstained with Harris Hematoxylin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
