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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 16231-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SKLFNHLSA VSESIQALGW VAMAPKPGPY VKEMNDAAMF YTNRVLKEYK DVDKKHVDWV KAYLSIWTEL QAYIKEFHTT GLAWSKTGPV AKELSGLPSG PSAGSGPPPP PPGPPPPPVS TSSGSDESAS RSALFAQINQ GESITHALKH VSDDMKTHKN PALKAQSGPV RSGPKPFSAP KPQTSPSPKR ATKKEPAVLE LEGKKWRVEN QENVSNLVIE DTELKQVAYI YKCVNTTLQI KGKINSITVD NCKKLGLVFD DVVGIVEIIN SKDVKVQVMG KVPTISINKT DGCHAYLSKN SLDCEIVSAK SSEMNVLIPT EGGDFNEFPV PEQFKTLWNG QKLVTTVTEI AG (125-475 aa encoded by BC013963)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skin tissue were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP ( CAP1 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP ( CAP1 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug CAP1 antibody (16231-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CAP1 (IP:16231-1-AP, 3ug; Detection:16231-1-AP 1:500) with A431 cells lysate 1520ug.
