HPA062087

antibody from Atlas Antibodies

Targeting: FDX1 ADX, FDX

Antibody data

Submit

Validation data

Product number: HPA062087 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA062087, RRID:AB_2630366
Product name: Anti-FDX1
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: WLHHAGSRAGSSGLLRNRGPGGSAEASRSLSVSAR ARSSSEDKITVHFINRDGETLTTKG
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

FDX1 protein structure - HPA062087 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human adrenal gland and cerebral cortex tissues using Anti-FDX1 antibody. Corresponding FDX1 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human adrenal gland, cerebral cortex, colon and lymph node using Anti-FDX1 antibody HPA062087 (A) shows similar protein distribution across tissues to independent antibody HPA041630 (B).
Antibody #2 product nr: HPA041630
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human adrenal gland shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of adrenal gland shows strong cytoplasmic positivity in cortical cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node using Anti-FDX1 antibody HPA062087.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human colon using Anti-FDX1 antibody HPA062087.
Sample type: HUMAN