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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX84451 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84451, RRID:AB_10728924
- Product name
- GBE1 antibody [4A1]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1 .
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-GBE1 mouse monoclonal antibody (GTX84451) immunofluorescent staining of COS7 cells transiently transfected with GBE1
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody (GTX84451).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation (IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-GBE1 mouse monoclonal antibody. (GTX84451, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-GBE1 antibody(GTX84451),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-GBE1 antibody(GTX84451),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
