Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 12527-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12527-1-AP, RRID:AB_10603358
- Product name
- CHMP2B antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CHMP2B antibody (Cat. #12527-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Human Cytomegalovirus Utilizes Extracellular Vesicles To Enhance Virus Spread.
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.
Allicin attenuated chronic social defeat stress induced depressive-like behaviors through suppression of NLRP3 inflammasome.
iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.
Characterization of Divalent Metal Transporter 1 (DMT1) in Brugia malayi suggests an intestinal-associated pathway for iron absorption.
Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.
Iron Regulatory Protein 1 Suppresses Hypoxia-Induced Iron Uptake Proteins Expression and Decreases Iron Levels in HepG2 Cells.
Streck NT, Zhao Y, Sundstrom JM, Buchkovich NJ
Journal of virology 2020 Jul 30;94(16)
Journal of virology 2020 Jul 30;94(16)
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.
Mercier V, Larios J, Molinard G, Goujon A, Matile S, Gruenberg J, Roux A
Nature cell biology 2020 Aug;22(8):947-959
Nature cell biology 2020 Aug;22(8):947-959
Allicin attenuated chronic social defeat stress induced depressive-like behaviors through suppression of NLRP3 inflammasome.
Gao W, Wang W, Liu G, Zhang J, Yang J, Deng Z
Metabolic brain disease 2019 Feb;34(1):319-329
Metabolic brain disease 2019 Feb;34(1):319-329
iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
Gao K, Zhang Y, Shi Q, Zhang J, Zhang L, Sun H, Jiao D, Zhao X, Tao H, Wei Y, Wang Y, Saiyin H, Zhao SM, Li Y, Zhang P, Wang C
Cell death & disease 2018 May 1;9(5):528
Cell death & disease 2018 May 1;9(5):528
VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.
Ott C, Nachmias D, Adar S, Jarnik M, Sherman S, Birnbaum RY, Lippincott-Schwartz J, Elia N
Scientific reports 2018 Feb 20;8(1):3353
Scientific reports 2018 Feb 20;8(1):3353
Characterization of Divalent Metal Transporter 1 (DMT1) in Brugia malayi suggests an intestinal-associated pathway for iron absorption.
Ballesteros C, Geary JF, Mackenzie CD, Geary TG
International journal for parasitology. Drugs and drug resistance 2018 Aug;8(2):341-349
International journal for parasitology. Drugs and drug resistance 2018 Aug;8(2):341-349
Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.
Huo Y, Zheng Z, Chen Y, Wang Q, Zhang Z, Deng H
Oncotarget 2016 Jul 19;7(29):45876-45888
Oncotarget 2016 Jul 19;7(29):45876-45888
Iron Regulatory Protein 1 Suppresses Hypoxia-Induced Iron Uptake Proteins Expression and Decreases Iron Levels in HepG2 Cells.
Cheng CM, Wang D, Cao X, Luo QQ, Lu YP, Zhu L
Journal of cellular biochemistry 2015 Sep;116(9):1919-31
Journal of cellular biochemistry 2015 Sep;116(9):1919-31
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12527-1-AP(CHMP2B antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CHMP2B antibody 12527-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 12527-1-AP(CHMP2B antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 12527-1-AP(CHMP2B antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue