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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00009021-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009021-M02, RRID:AB_733247
- Product name
- SOCS3 monoclonal antibody (M02), clone 1E4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SOCS3.
- Antigen sequence
MVTHSKFPAAGMSRPLDTSLRLKTFSSKSEYQLVV
NAVRKLQESGFYWSAVTGGEANLLLSAEPAGTFLI
RDSSDQRHFFTLSVKTQSGTKNLRIQCEGGSFSLQ
SDPRSTQPVPRFDCVLKLVHHYMPPPGAPSFPSPP
TEPSSEVPEQPSAQPLPGSPPRRAYYIYSGGEKIP
LVLSRPLSSNVATLQHLCRKTVNGHLDSYEKVTQL
PGPIREFLDQYDAPL- Isotype
- IgG
- Antibody clone number
- 1E4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Epigenetic silencing of SOCS3 identifies a subset of prostate cancer with an aggressive behavior.
Pierconti F, Martini M, Pinto F, Cenci T, Capodimonti S, Calarco A, Bassi PF, Larocca LM
The Prostate 2011 Feb 15;71(3):318-25
The Prostate 2011 Feb 15;71(3):318-25
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SOCS3 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to SOCS3 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to SOCS3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
