Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00006237-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006237-M01, RRID:AB_547894
- Product name
- Mouse Monoclonal RRAS Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RRAS - related RAS viral (r-ras) oncogene homolog
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Prolonged activation of cAMP signaling leads to endothelial barrier disruption via transcriptional repression of RRAS.
Perrot CY, Sawada J, Komatsu M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 May 18;:fj201700818RRR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 May 18;:fj201700818RRR
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RRAS Antibody (2E12) [H00006237-M01] - RRAS monoclonal antibody (M01), clone 2E12 Analysis of RRAS expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RRAS Antibody (2E12.) [H00006237-M01] - RRAS Antibody (2E12) [H00006237-M01] - Detection limit for recombinant GST tagged RRAS is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RRAS Antibody (2E12) [H00006237-M01] - Analysis of RRAS transfected lysate using anti-RRAS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RRAS MaxPab rabbit polyclonal antibody.