27457-1-AP

antibody from Invitrogen Antibodies

Targeting: RRAS R-Ras

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

27457-1-AP

Provider

Invitrogen Antibodies

Product name

RRAS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DLESQRQVP RSEASAFGAS HHVAYFEASA KLRLNVDEAF EQLVRAVRKY QEQELPPSPP SAPRKKGGGC PCVLL (145-218 aa encoded by BC016318)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.4 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HT-29 cells were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using (Product # 27457-1-AP) (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).