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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB2486 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2486, RRID:AB_1577577
- Product name
- NANOS1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NANOS1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references E-cadherin regulates human Nanos1, which interacts with p120ctn and induces tumor cell migration and invasion.
Time-lapse analysis reveals different modes of primordial germ cell migration in the medaka Oryzias latipes.
Strumane K, Bonnomet A, Stove C, Vandenbroucke R, Nawrocki-Raby B, Bruyneel E, Mareel M, Birembaut P, Berx G, van Roy F
Cancer research 2006 Oct 15;66(20):10007-15
Cancer research 2006 Oct 15;66(20):10007-15
Time-lapse analysis reveals different modes of primordial germ cell migration in the medaka Oryzias latipes.
Kurokawa H, Aoki Y, Nakamura S, Ebe Y, Kobayashi D, Tanaka M
Development, growth & differentiation 2006 Apr;48(3):209-21
Development, growth & differentiation 2006 Apr;48(3):209-21
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NANOS1 polyclonal antibody (Cat # PAB2486) in T-47D cell line lysates (35 ug/lane). NANOS1 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NANOS1 polyclonal antibody (Cat # PAB2486) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 polyclonal antibody (Cat # PAB2486) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using NANOS1 polyclonal antibody (Cat # PAB2486)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
