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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-47701 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-47701, RRID:AB_677353
- Product name
- Anti-POLR2A
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Evolutionary conserved regulation of HIF-1β by NF-κB.
The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing.
HIF-1α depletion results in SP1-mediated cell cycle disruption and alters the cellular response to chemotherapeutic drugs.
Mechanism of hypoxia-induced NF-kappaB.
Adenomatous polyposis coli and hypoxia-inducible factor-1{alpha} have an antagonistic connection.
Halogenated imidazole derivatives block RNA polymerase II elongation along mitogen inducible genes.
Comprehensive analysis of the palindromic motif TCTCGCGAGA: a regulatory element of the HNRNPK promoter.
FACT facilitates chromatin transcription by RNA polymerases I and III.
van Uden P, Kenneth NS, Webster R, Müller HA, Mudie S, Rocha S
PLoS genetics 2011 Jan 27;7(1):e1001285
PLoS genetics 2011 Jan 27;7(1):e1001285
The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing.
Brody Y, Neufeld N, Bieberstein N, Causse SZ, Böhnlein EM, Neugebauer KM, Darzacq X, Shav-Tal Y
PLoS biology 2011 Jan 11;9(1):e1000573
PLoS biology 2011 Jan 11;9(1):e1000573
HIF-1α depletion results in SP1-mediated cell cycle disruption and alters the cellular response to chemotherapeutic drugs.
Culver C, Melvin A, Mudie S, Rocha S
Cell cycle (Georgetown, Tex.) 2011 Apr 15;10(8):1249-60
Cell cycle (Georgetown, Tex.) 2011 Apr 15;10(8):1249-60
Mechanism of hypoxia-induced NF-kappaB.
Culver C, Sundqvist A, Mudie S, Melvin A, Xirodimas D, Rocha S
Molecular and cellular biology 2010 Oct;30(20):4901-21
Molecular and cellular biology 2010 Oct;30(20):4901-21
Adenomatous polyposis coli and hypoxia-inducible factor-1{alpha} have an antagonistic connection.
Newton IP, Kenneth NS, Appleton PL, Näthke I, Rocha S
Molecular biology of the cell 2010 Nov 1;21(21):3630-8
Molecular biology of the cell 2010 Nov 1;21(21):3630-8
Halogenated imidazole derivatives block RNA polymerase II elongation along mitogen inducible genes.
Mikula M, Hanusek K, Paziewska A, Dzwonek A, Rubel T, Bomsztyk K, Ostrowski J
BMC molecular biology 2010 Jan 15;11:4
BMC molecular biology 2010 Jan 15;11:4
Comprehensive analysis of the palindromic motif TCTCGCGAGA: a regulatory element of the HNRNPK promoter.
Mikula M, Gaj P, Dzwonek K, Rubel T, Karczmarski J, Paziewska A, Dzwonek A, Bragoszewski P, Dadlez M, Ostrowski J
DNA research : an international journal for rapid publication of reports on genes and genomes 2010 Aug;17(4):245-60
DNA research : an international journal for rapid publication of reports on genes and genomes 2010 Aug;17(4):245-60
FACT facilitates chromatin transcription by RNA polymerases I and III.
Birch JL, Tan BC, Panov KI, Panova TB, Andersen JS, Owen-Hughes TA, Russell J, Lee SC, Zomerdijk JC
The EMBO journal 2009 Apr 8;28(7):854-65
The EMBO journal 2009 Apr 8;28(7):854-65
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 217
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
