664911

antibody from BioLegend

Targeting: POLR2A POLR2, POLRA, RPB1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

664911

Provider

BioLegend

Proper citation

BioLegend Cat#664911, RRID:AB_2650947

Product name

Go-ChIP-Grade™ Purified anti-RNA Polymerase II RPB1

Antibody type

Monoclonal

Reactivity

Human, Mouse, Yeast

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

8WG16

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from HeLa cells with either A) 1:300 dilution of Go-ChIP-Grade™ Purified anti-RNA Polymerase II Antibody (Clone 8WG16), B) equal amount of Purified Mouse IgG2a Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-RNA Polymerase II Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.

Conjugate

Unconjugated