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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- V7525 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- POLR2A Antibody / RNA polymerase II subunit RPB1 / Phosphorylated Serine 5
- Antibody type
- Monoclonal
- Description
- PEG precipitation
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the phospho-RNA polymerase II antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Submitted references Highly asynchronous and asymmetric cleavage divisions accompany early transcriptional activity in pre-blastula medaka embryos.
Prolyl isomerase Pin1 shares functional similarity with phosphorylated CTD interacting factor PCIF1 in vertebrate cells.
Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation.
Hyperphosphorylation of RNA polymerase II in response to topoisomerase I cleavage complexes and its association with transcription- and BRCA1-dependent degradation of topoisomerase I.
FLASH acts as a co-activator of the transcription factor c-Myb and localizes to active RNA polymerase II foci.
Purification of elongating RNA polymerase II and other factors from yeast chromatin.
Kraeussling M, Wagner TU, Schartl M
PloS one 2011;6(7):e21741
PloS one 2011;6(7):e21741
Prolyl isomerase Pin1 shares functional similarity with phosphorylated CTD interacting factor PCIF1 in vertebrate cells.
Yunokuchi I, Fan H, Iwamoto Y, Araki C, Yuda M, Umemura H, Harada F, Ohkuma Y, Hirose Y
Genes to cells : devoted to molecular & cellular mechanisms 2009 Sep;14(9):1105-18
Genes to cells : devoted to molecular & cellular mechanisms 2009 Sep;14(9):1105-18
Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation.
Li OT, Chan MC, Leung CS, Chan RW, Guan Y, Nicholls JM, Poon LL
PloS one 2009 May 21;4(5):e5658
PloS one 2009 May 21;4(5):e5658
Hyperphosphorylation of RNA polymerase II in response to topoisomerase I cleavage complexes and its association with transcription- and BRCA1-dependent degradation of topoisomerase I.
Sordet O, Larochelle S, Nicolas E, Stevens EV, Zhang C, Shokat KM, Fisher RP, Pommier Y
Journal of molecular biology 2008 Sep 5;381(3):540-9
Journal of molecular biology 2008 Sep 5;381(3):540-9
FLASH acts as a co-activator of the transcription factor c-Myb and localizes to active RNA polymerase II foci.
Alm-Kristiansen AH, Saether T, Matre V, Gilfillan S, Dahle O, Gabrielsen OS
Oncogene 2008 Aug 7;27(34):4644-56
Oncogene 2008 Aug 7;27(34):4644-56
Purification of elongating RNA polymerase II and other factors from yeast chromatin.
Svejstrup JQ, Petrakis TG, Fellows J
Methods in enzymology 2003;371:491-8
Methods in enzymology 2003;371:491-8
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HepG2, K562, and SK-BR-3 cell lysate with phospho-RNA polymerase II antibody (clone CTD 8A7). Routinely observed molecular weight: 200-250 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colon carcinoma with phospho-RNA polymerase II antibody (clone CTD 8A7). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colon carcinoma with phospho-RNA polymerase II antibody (clone CTD 8A7). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
