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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 17-620 - Provider product page
- Provider
- EMD Millipore
- Proper citation
- Millipore Cat#17-620, RRID:AB_11215103
- Product name
- ChIPAb+ RNA Pol II - ChIP Validated Antibody and Primer Set
- Antibody type
- Monoclonal
- Antigen
- The RNA Pol II antibody is made against a peptide corresponding to the C-terminal domain of RNA Pol II.
- Reactivity
- Human, Mouse, Rat, Yeast
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 25 assays
- Storage
- Stable for 1 year at -20°C from date of receipt.
Submitted references Discovery of SMAD4 promoters, transcription factor binding sites and deletions in juvenile polyposis patients.
Calva D, Dahdaleh FS, Woodfield G, Weigel RJ, Carr JC, Chinnathambi S, Howe JR
Nucleic acids research 2011 Jul;39(13):5369-78
Nucleic acids research 2011 Jul;39(13):5369-78
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Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- 0.05-1 _g/mL of this lot detected RNA polymerase II in HeLa nuclear extracts.
- Validation comment
- WB
Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- Sonicated chromatin prepared from untreated HeLa cells was subjected to chromatin immunoprecipitation using 1 µg of either a negative control antibody or Mouse Anti-RNA Pol II and the Magna ChIPª G Kit (Cat. # 17-611) with overnight IP. Successful immunoprecipitation of RNA Pol II associated DNA fragments was verified by qPCR using Control Primers flanking a binding site in the human GAPDH promoter
- Validation comment
- ChIP
